Gene Regulation Sequencing

RNA-Seq (Quantification)

By enrichment of all mRNA, randomly splicing into small species and sequencing from single end that could get the expression level highly efficient.

Feature : higher accuracy compare to microarray

Sequencing output: 10M or 20M
 

Transcriptome

Transcriptome assembly is the method of studying gene expression and structure in a specific developmental stage or physiological condition. Transcriptome sequencing could be applied on fundamental science research, medical research.

Feature : quantification, detecting new transcripts , alternative splices & fusion genes

Sequencing output : 4G
 

Small RNA

Small RNAs are including miRNA、siRNA、piRNA by sequencing specific silence could regulate translation and physiological process. Small RNA sequencing could study the composition and function of small RNA in a special condition.

Feature : detect novel miRNA,predict miRNA target gene.

Sequencing output : 5M or 10M


ChIP-Sequencing

Chromatin IP is a technique to study the interactions between protein and DNA in vivo. ChIP-sequencng could identify the binding sites of DNA associated protein accuratly and applied to transcription factors regulation.

Feature : collect all information of transcription regulation and protein modification

Sequencing output : 20M
 

MeDIP-Sequencing

MeDIP is based on enrichment the high methylation region of DNA fragment and sequencing the methylation area. Comparing DNA methylation modification pattens between different cells or tissues could offer the information for disease study.

Feature

a. High coverage : covers whole genome methylation area
b. Cost effective
Sequencing output : 5G