Third Generation Sequencing

Whole Genome Sequencing

PacBio Sequencing Platform delivers long read lengths (average 10~12kb, longest >60kb) with the highest consensus accuracy and uniform coverage, which generate the most comprehensive de novo assemblies meanwhile provides complete and accurate views of all types of genomic variation, revealing SNPs/SNVs, structural variants, mobile elements, haplotypes, epigenetics, and variants in low-complexity regions.

Microbial Whole Genome Sequencing 

Only need a SMRT cell to complete a bacterial genome assembly, fully achieve the best
assembly results “One Genome, One Contig” and get epigenetic information at the same time.

Plant and Animal Whole Genome Sequencing 

Longer read length can achieve the best condition for assemblies. These comprehensive assemblies capture undetected SNPs, fully intact genes, and regulatory regions embedded in complex structures that fragmented draft genomes often miss.

  • Gain insight into individual gene functions and their coordination within networks
  • Obtain intact genes and gene clusters for reconstructing biosynthetic pathways
  • Differentiate and distinguish gene expansion and duplication events to understand adaptive mechanisms for selection pressure
  • Resolve transposable elements for evolutionary analysis to trace adaptive, retained, and transferred traits

Human Whole Genome Sequencing

There is a growing awareness that SNVs do not tell the whole story of human genetic diversity, and that structural variation can be an important driver of health and disease susceptibility. In fact, the majority of variant bases in the human genome occur in structural variants. We can provide the longest reads length to gain access all variant types of genomes.

Features

a.Provides the longest reads
b.Uniform coverage
c.Highest consensus accuracy
d.Generating high quality reference genome & contiguous assemblies
 

Targeted Sequencing

Targeted sequencing is a powerful way to increase the cost-effectiveness of variant discovery and detection. By long-read technology, the entire length of the fragment can be sequenced at one time, avoiding mis-mapping, PCR-related bias and span repeats.

Features

a. Provides Phasing results accurately.
b. High coverage in GC-rich region


RNA Sequencing

The isoform sequencing (Iso-Seq) application generates full-length cDNA sequences — from the 5’ end of transcripts to the poly-A tail — eliminating the need for transcriptome reconstruction using isoform-inference algorithms. The Iso-Seq method generates accurate information about alternatively spliced exons and transcriptional start sites. It also delivers information about poly-adenylation sites for transcripts up to 10 kb in length across the full complement of isoforms within targeted genes or the entire transcriptome.

Features

a. Application of allele-specific gene expression
b. Whole length transcripts sequencing directly