Pirmer degraded(N-1.N-2…)

Status descriptions

It detects smaller peaks at the lower part of the image, which are one base away from the main peaks. The phenomenon is called “shift”.

Possible reasons

The primer used for sequencing degrades, and produces primer mixture in different lengths. This may cause non-specific binding.

Solutions

Avoid repetitive thawing and freezing. During sequencing, it is suggested to use freshly prepared primer.