It appears disordered signals after a string of consecutive mononucleotides

Status descriptions

  • It appears disordered signals after a string of consecutive mononucleotides
  • Such situation may also occur after a string of repetitive dinucleotides

Possible reasons

Loops subject to internal binding can form during the DNA extension step of PCR

Solutions

  • Make sequencing again from the primer at the opposite end.
  • Neglect the sequence of consecutive mononucleotides and design another primer to make sequencing.