The signal is weak or has disordered background

Status descriptions

  • It appears other weak peaks under the main peaks.
  • The signal value is lower than 150 before 300 bp.

Possible reasons

  • The sequencing reaction fails.
  • There are too little template DNAs.
  • The products lose when during the purification of the sequencing products.
  • The used primer is not adhesive enough.


Solutions are the same with the improvement methods for “sequencing failed”.