The signal is illegible

Status descriptions

  • The peak looks round and wide before 500 bp.
  • There is no obvious segregation between two signals.

Possible reasons

  • The capillary electrophoresis exceeds the load. The residual salt and proteins in the sample make the peak resolution poor and make it unable to show sharp and obvious wave peaks.
  • The voltage of the capillary electrophoresis is too high.
  • The used buffer or electrophoresis gel (polymer) is in bad quality.

Solutions

  • Check whether the sample is in good quality.
  • Check the voltage of the capillary electrophoresis.
  • Check whether the quality of the used buffer or gel (polymer) is stable.