DNA Methylation Analysis
DNA methylation is an epigenetic mechanism that often affects the expression of genes. It is important in numerous cellular processes, including embryonic development, genomic imprinting, human diseases, environmental hormones effects.
Service Platform
Pyrosequence
Instrument：QIAGEN PyroMark Q24
Workflow：
- DNA Bisulfate CT conversion
- BSPCR
- sequencing
- Quality control and software analysis
- Report
Advantages：
- Outstanding sensitivity compared with traditional sequencing method.
- Quantification of individual CpG sites
- Multi-loci analysis
- Providing effectiveness and reproducibility of experiments by QIAGEN kit
EpiTect Methyl II QPCR Array
Instrument ：ABI ViiA 7
Workflow：
- Cleavage DNA with a methylation-sensitive and/or a methylation-dependent restriction enzyme
- QIAGEN SABioscience EpiTect Methyl II QPCR Array/Assay
- Perform Thermal Cycling (amplification data and melting curve)
- software analysis （convert Ct values to Met%）
- Report
Advantages：
- Profiling of 22 or 94 genes in 96- or 384-well formats.
- Providing effectiveness and reproducibility of experiments by QIAGEN kit