Life Science

DNA Methylation Analysis

DNA methylation is an epigenetic mechanism that often affects the expression of genes. It is important in numerous cellular processes, including embryonic development, genomic imprinting, human diseases, environmental hormones effects.

Service Platform

Pyrosequence

Instrument:QIAGEN PyroMark Q24

Workflow:

  • DNA Bisulfate CT conversion
  • BSPCR
  • sequencing
  • Quality control and software analysis
  • Report

Advantages:

  • Outstanding sensitivity compared with traditional sequencing method.
  • Quantification of individual CpG sites 
  • Multi-loci analysis
  • Providing effectiveness and reproducibility of  experiments by QIAGEN kit 

 

EpiTect Methyl II QPCR Array

Instrument :ABI ViiA 7

Workflow:

  • Cleavage DNA with a methylation-sensitive  and/or a methylation-dependent restriction enzyme
  • QIAGEN SABioscience EpiTect Methyl II QPCR Array/Assay
  • Perform Thermal Cycling (amplification data and melting curve)
  • software analysis (convert Ct values to Met%)
  • Report

Advantages:

  • Profiling of  22 or 94 genes in 96- or 384-well formats.
  • Providing effectiveness and reproducibility of  experiments by QIAGEN kit