Life Science

Genotyping Analysis

Genotyping of SNP (single nucleotide polymorphism) / STR (short tandem repeat) is applied widely in diagnosis of genetic disease, mutation detection, human identification, preventive medicine, drug dose Prediction and so on.

SNP Genotyping Analysis

Sanger Sequencing

Instrument:ABI 3730XL DNA Analyzer

Workflow:

  • Primer design and systhesis
  • Target sequence PCR
  • Quality control
  • Purification and sequencing
  • Software analysis
  • Report

Advantages :

Read length ~800bp, suitable for multi-loci analysis


 

Pyrosequencing

Instrument:QIAGEN PyroMark Q24

Workflow:

  • Primer design and systhesis
  • Target sequence PCR
  • Pyrosequencing
  • Software analysis
  • Report

Advantages:

Percentage of wild and mutant type, detailed sequence information


 

SNP Genotyping Analysis

TaqMan Allelic Discrimination

Instrument :ABI ViiA 7

Workflow:

  • Probe purchasing
  • TaqMan QPCR
  • Software analysis
  • Report

Advantages:

numerous samples


 

STR Genotyping Analysis

DNA Fragment Analysis

Instrument : 3730XL DNA Analyzer

Workflow:

  • Primer design and systhesis
  • Target sequence PCR
  • Quality control
  • Capillary electrophoresis (3730XL DNA Analyzer)
  • GeneMapper v4.0 software analysis
  • Report