Genotyping Analysis
Genotyping of SNP (single nucleotide polymorphism) / STR (short tandem repeat) is applied widely in diagnosis of genetic disease, mutation detection, human identification, preventive medicine, drug dose Prediction and so on.
SNP Genotyping Analysis
Sanger Sequencing
Instrument:ABI 3730XL DNA Analyzer
Workflow:
- Primer design and systhesis
- Target sequence PCR
- Quality control
- Purification and sequencing
- Software analysis
- Report
Advantages :
Read length ~800bp, suitable for multi-loci analysis
Pyrosequencing
Instrument:QIAGEN PyroMark Q24
Workflow:
- Primer design and systhesis
- Target sequence PCR
- Pyrosequencing
- Software analysis
- Report
Advantages:
Percentage of wild and mutant type, detailed sequence information
SNP Genotyping Analysis
TaqMan Allelic Discrimination
Instrument :ABI ViiA 7
Workflow:
- Probe purchasing
- TaqMan QPCR
- Software analysis
- Report
Advantages:
numerous samples
STR Genotyping Analysis
DNA Fragment Analysis
Instrument : 3730XL DNA Analyzer
Workflow:
- Primer design and systhesis
- Target sequence PCR
- Quality control
- Capillary electrophoresis (3730XL DNA Analyzer)
- GeneMapper v4.0 software analysis
- Report